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PCR products were purified using a QIAquick gel extraction kit (Qiagen, CA, USA) followed by electrophoresis on 1.5% agarose gels. Five hundred nanograms of the purified PCR products were sequenced by the Shanghai Sangon Company (Sangon, Shanghai, China) on an ABI 373 automated sequencer (Applied Biosystems, CA, USA). The sequence data were analysed using the Sequencer program, version 3.0. DNAssist version 1.02 was used to compare the sequences with the H37Rv genome database (GenBank accession no. L27989).. obtained within 24 h and requires less than 2 h of actual hands-on

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Serum ubiquitin C-terminal hydrolase L1 (UCH-L1) has been proposed as a biomarker of traumatic brain injury (TBI). However, previous studies on levels of UCH-L1 in serum remain inconsistent. This systematic review and meta-analysis were conducted on observational studies that reported the association between serum UCH-L1 levels and TBI.. Overview. Although none of the subjects in either of the two geographic areas was selenium deficient, the level of plasma selenium was significantly lower in men from the high-risk area compared with those from the low-risk area. Levels of selenoprotein-P and glutathione peroxidase activity were similar between groups after adjustment for confounders. Selenium measurements were not associated with histopathological diagnosis.. in addition to the consensus method. SMM-align is a matrix-based.

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